Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
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چکیده
منابع مشابه
Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
Whole transcriptome sequencing by mRNA-Seq is now used extensively to perform global gene expression, mutation, allele-specific expression and other genome-wide analyses. mRNA-Seq even opens the gate for gene expression analysis of non-sequenced genomes. mRNA-Seq offers high sensitivity, a large dynamic range and allows measurement of transcript copy numbers in a sample. Illumina's genome analy...
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MOTIVATION The Illumina paired-end sequencing technology can generate reads from both ends of target DNA fragments, which can subsequently be merged to increase the overall read length. There already exist tools for merging these paired-end reads when the target fragments are equally long. However, when fragment lengths vary and, in particular, when either the fragment size is shorter than a si...
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Many investigators who do high-throughput sequencing of mRNA (mRNA-Seq) use kits for library preparation purchased from Illumina. Recognizing that these kits are continually being updated and improved, we provide here some background information, tips, and troubleshooting advice for the kits available at the time of this writing.
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2011
ISSN: 1940-087X
DOI: 10.3791/3340